1MGO
Horse Liver Alcohol Dehydrogenase Phe93Ala Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-07-25 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.144, 51.308, 93.481 |
| Unit cell angles | 91.98, 103.01, 109.81 |
Refinement procedure
| Resolution | 20.000 - 1.200 |
| R-factor | 0.18772 |
| Rwork | 0.188 |
| R-free | 0.21000 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hld |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.840 * |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.27) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.240 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.073 | 0.290 |
| Total number of observations | 455785 * | |
| Number of reflections | 199442 | |
| <I/σ(I)> | 5.3 | 1.5 |
| Completeness [%] | 86.6 * | 84.5 |
| Redundancy | 2.25 | 1.46 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microdialysis * | 6.7 * | 253 * | MPD, pH 7.0, Dialysis, temperature 277K |
| 1 | Microdialysis * | 6.7 * | 253 * | MPD, pH 7.0, Dialysis, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | enzyme | 26 (mg/ml) | |
| 2 | 1 | 2 | MPD | 13 (%) | |
| 3 | 1 | 2 | ammmonium N-[Tris(hydroxymethyl)methyl]-2-aminoethanesulfonate buffer | 50 (mM) | |
| 4 | 1 | 2 | EDTA | 0.25 (mM) | pH6.7 |
| 5 | 1 | 2 | NAD+ | 20 (mM) | |
| 6 | 1 | 2 | pentafluorobenzyl alcohol | 100 (mM) |
