1MGN

HIS64(E7)-> TYR APOMYOGLOBIN AS A REAGENT FOR MEASURING RATES OF HEMIN DISSOCIATION

Experimental procedure

Spacegroup name	P 6
Unit cell lengths	91.200, 91.200, 45.870
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	? - 1.900
R-factor	0.153
Rwork	0.153
RMSD bond length	0.022
RMSD bond angle	2.570
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	1.800 *
Rmerge	0.051 *
Completeness [%]	91.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	9 *	20 *	taken from Phillips, G.N. et al (1990). Proteins. Struct. Funct.Genet., 7, 308. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	ammonium sulfate	2.6 (M)
3	1	reservoir	Tris-HCl	20 (mM)
4	1	reservoir	EDTA	1 (mM)