1MG7
Crystal Structure of xol-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2001-11-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 116.071, 86.023, 80.240 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.550 |
| Rwork | 0.204 |
| R-free | 0.21300 * |
| Structure solution method | MAD |
| RMSD bond length | 0.006 * |
| RMSD bond angle | 1.400 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.550 |
| High resolution limit [Å] | 1.550 | 1.500 |
| Rmerge | 0.054 * | 0.540 * |
| Number of reflections | 113135 * | 12340 * |
| <I/σ(I)> | 25.2 | 1.6 |
| Completeness [%] | 97.3 | 96.9 * |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.8 | 4 * | peg 400, magnesium chloride, hepes, dtt, pH 7.8, VAPOR DIFFUSION, SITTING DROP, temperature 277.15K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 80 (mg/ml) | |
| 2 | 1 | reservoir | HEPES | 0.1 (M) | pH7.8 |
| 3 | 1 | reservoir | 0.2 (M) | ||
| 4 | 1 | reservoir | PEG400 | 17.5 (%) | |
| 5 | 1 | reservoir | MPD | 5 (%) |
