1MG4

STRUCTURE OF N-TERMINAL DOUBLECORTIN DOMAIN FROM DCLK: WILD TYPE PROTEIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2002-03-08
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9187
Spacegroup name	C 1 2 1
Unit cell lengths	85.981, 29.621, 40.332
Unit cell angles	90.00, 101.33, 90.00

Refinement procedure

Resolution	20.000 * - 1.500 *
R-factor	0.15472
Rwork	0.153
R-free	0.18700 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mfw
RMSD bond length	0.016
RMSD bond angle	1.700 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.550
High resolution limit [Å]	1.500	1.500
Rmerge	0.084	0.286
Total number of observations	47701 *
Number of reflections	15460
<I/σ(I)>	14.1	2.5
Completeness [%]	96.8	85.7
Redundancy	3.1	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	21 *	CITRATE BUFFER, AMMONIUM SULFATE, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 294K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	ammonium sulfate	1.8-2.0 (M)
3	1	reservoir	sodium citrate	0.1 (M)	pH5.0