1MFT
Crystal Structure Of Four-Helix Bundle Model
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-10-31 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.605 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 25.233, 54.810, 37.168 |
Unit cell angles | 90.00, 105.08, 90.00 |
Refinement procedure
Resolution | 50.000 * - 2.500 |
R-factor | 0.222 |
Rwork | 0.222 |
R-free | 0.26600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ec5 |
RMSD bond length | 0.008 |
RMSD bond angle | 17.600 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.570 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.100 | 0.217 |
Total number of observations | 7203 * | |
Number of reflections | 3229 | |
<I/σ(I)> | 6.5 | 3 |
Completeness [%] | 93.8 | 97.8 |
Redundancy | 2 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 6 * | 291 | PEG 300, zinc chloride, bis-tris, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 2 (mg/ml) | |
2 | 1 | reservoir | PEG300 | 26 (%(w/v)) | |
3 | 1 | reservoir | twofold excess | ||
4 | 1 | reservoir | HEPES | 0.1 (M) | pH6.0 |