1MFM
MONOMERIC HUMAN SOD MUTANT F50E/G51E/E133Q AT ATOMIC RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-11-01 |
| Detector | MAR scanner 180 mm plate |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.990, 48.110, 81.080 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.020 |
| R-factor | 0.118 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sos |
| RMSD bond length | 0.031 |
| RMSD bond angle | 0.035 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | SHELXL |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.040 |
| High resolution limit [Å] | 1.000 | 1.020 |
| Rmerge | 0.061 * | 0.247 * |
| Total number of observations | 681833 * | |
| Number of reflections | 69841 * | |
| <I/σ(I)> | 9.7 | 2.2 |
| Completeness [%] | 99.1 | 98 |
| Redundancy | 4.5 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | 4 * | PEG 6000 15%, CDCL2 200-400 MM, TRIS 100MM, PH=8, pH 8.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | |
| 3 | 1 | reservoir | PEG6000 | 15 (%(v/v)) | |
| 4 | 1 | reservoir | 200-400 (mM) | ||
| 5 | 1 | reservoir | Tris-HCl | 100 (mM) |
