1MFL
The Structure of ERBIN PDZ domain bound to the Carboxy-terminal tail of the ErbB2 Receptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-06-04 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 26.526, 56.995, 30.951 |
| Unit cell angles | 90.00, 99.16, 90.00 |
Refinement procedure
| Resolution | 30.600 * - 1.880 |
| R-factor | 0.169 |
| Rwork | 0.167 |
| R-free | 0.21600 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mfg |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.579 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.600 | 1.950 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmerge | 0.028 | 0.096 |
| Number of reflections | 7068 | |
| <I/σ(I)> | 44.7 | 13.3 |
| Completeness [%] | 95.0 | 90.2 |
| Redundancy | 3.73 | 3.34 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.3 * | 20 * | 23-26% PEG 4000, 20% GLYCEROL, 100mM AMMONIUM ACETATE, 100mM SODIUM ACETATE, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 19 (mg/ml) | |
| 2 | 1 | drop | 500 (mM) | ||
| 3 | 1 | drop | Tris-HCl | 50 (mM) | pH8.3 |
| 4 | 1 | reservoir | PEG4000 | 12-15 (%) | |
| 5 | 1 | reservoir | glycerol | 10 (%) | |
| 6 | 1 | reservoir | ammonium acetate | 100 (mM) | |
| 7 | 1 | reservoir | sodium acetate | 100 (mM) | pH4.6 |
