1MFD
THE SOLUTION STRUCTURE OF A TRISACCHARIDE-ANTIBODY COMPLEX: COMPARISON OF NMR MEASUREMENTS WITH A CRYSTAL STRUCTURE
Experimental procedure
Spacegroup name | P 21 21 21 |
Unit cell lengths | 47.300, 129.300, 79.900 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 * - 2.100 |
R-factor | 0.183 |
Rwork | 0.183 |
RMSD bond length | 0.013 |
RMSD bond angle | 3.100 |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
High resolution limit [Å] | 2.100 * |
Rmerge | 0.047 * |
Total number of observations | 86916 * |
Number of reflections | 24335 * |
Completeness [%] | 83.0 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8 * | drops were prepared by mixing 0.003 ml of Fab and 0.004 ml of well solution with 0.001 ml of triaccharide * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | Fab | 7.3 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 10 (mM) | |
3 | 1 | drop | 0.1 (M) | ||
4 | 1 | drop | trisaccharide | 10 (mg/ml) | |
5 | 1 | reservoir | PEG8000 | 15 (%) | |
6 | 1 | reservoir | Tris-HCl | 50 (mM) |