1MF7

INTEGRIN ALPHA M I DOMAIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Spacegroup name	P 21 21 21
Unit cell lengths	38.640, 51.030, 102.380
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.250
R-factor	0.166
Rwork	0.166
R-free	0.22300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012 *
RMSD bond angle	2.300 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	SHELXL

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	1.250
Rmerge	0.028 *	0.234 *
Total number of observations	170186 *
Number of reflections	53821
Completeness [%]	94.6 *	89.4 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	8 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop		100 (mM)
2	1	drop		5 (mM)
3	1	drop	Tris	20 (mM)	pH8.0
4	1	drop	protein	10 (mg/ml)
5	1	reservoir	PEG2000MME	22-30 (%(w/v))
6	1	reservoir	ammonium sulfate	0.2 (M)
7	1	reservoir	sodium acetate	0.1 (M)	pH4.6