1MF5
GCATGCT Quadruplex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-05-19 |
Detector | MARRESEARCH |
Wavelength(s) | 0.811 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 22.128, 59.215, 45.784 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.100 |
R-factor | 0.216 |
Rwork | 0.213 |
R-free | 0.22400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | UDG028 |
RMSD bond length | 0.018 |
RMSD bond angle | 0.035 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SHELX |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 * | 1.200 |
High resolution limit [Å] | 1.100 | 1.100 |
Rmerge | 0.110 | 0.370 |
Total number of observations | 12514 * | |
Number of reflections | 2803 * | |
<I/σ(I)> | 10.96 | 5.24 |
Completeness [%] | 99.3 | 99.4 |
Redundancy | 7.70 * | 6.64 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.6 | 290 * | Cobalt Hexammine, Cacodylate, MPD, NaCl, KCl, pH 6.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | Cobalt Hexammine | ||
2 | 1 | 1 | Cacodylate | ||
3 | 1 | 1 | MPD | ||
4 | 1 | 1 | NaCl | ||
5 | 1 | 1 | KCl |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | sodium cacodylate | 40 (mM) | |
2 | 1 | drop | cobalt hexammine | 80 (mM) | |
3 | 1 | drop | 10 (%) | ||
4 | 1 | drop | 800 (mM) | ||
5 | 1 | drop | 30 (mM) | ||
6 | 1 | drop | DNA | 1 (mM) | |
7 | 1 | reservoir | MPD | 30 (%) |