1MF2
ANTI HIV1 PROTEASE FAB COMPLEX
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-11-03 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.600, 94.700, 70.300 |
| Unit cell angles | 90.00, 105.80, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.600 |
| R-factor | 0.206 |
| Rwork | 0.206 |
| R-free | 0.30700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fbi |
| RMSD bond length | 0.011 |
| RMSD bond angle | 28.140 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.106 * | 0.300 |
| Total number of observations | 105230 * | |
| Number of reflections | 25505 | |
| <I/σ(I)> | 10 | 1.5 |
| Completeness [%] | 96.8 | 93.4 |
| Redundancy | 4.2 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 | 17 * | pH 8.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Fab | 6.5 (mg/ml) | |
| 2 | 1 | drop | PEG4000 | 10 (%) | |
| 3 | 1 | drop | sodium citrate | 0.05 (M) | |
| 4 | 1 | reservoir | PEG4000 | 20 (%) | |
| 5 | 1 | reservoir | sodium citrate | 0.1 (M) |
