1MEX
Antibody Catalysis of a Bimolecular Cycloaddition Reaction
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 84 |
Detector technology | CCD |
Collection date | 2001-05-06 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.96482 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.320, 83.310, 107.180 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.250 |
Rwork | 0.153 |
R-free | 0.21310 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 0.034 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.280 |
High resolution limit [Å] | 1.250 | 1.250 |
Number of reflections | 112412 | |
<I/σ(I)> | 27.4 | 1.9 |
Completeness [%] | 99.5 | 99.5 |
Redundancy | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295.5 | MPEG2000, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K |