1MEL
CRYSTAL STRUCTURE OF A CAMEL SINGLE-DOMAIN VH ANTIBODY FRAGMENT IN COMPLEX WITH LYSOZYME
Experimental procedure
Source type | ROTATING ANODE |
Source details | OTHER |
Temperature [K] | 298 |
Detector technology | DIFFRACTOMETER |
Collection date | 1996-05-02 |
Detector | ENRAF-NONIUS FAST |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 68.680, 69.710, 113.680 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.500 |
R-factor | 0.221 |
Rwork | 0.221 |
R-free | 0.32300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 1LYS AND 1IGM |
RMSD bond length | 0.013 |
RMSD bond angle | 24.049 * |
Data reduction software | MADNES |
Data scaling software | SCALA ((CCP4)) |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 10.000 | 2.560 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.114 | 0.420 |
Number of reflections | 17829 | |
<I/σ(I)> | 6.1 | 1.8 |
Completeness [%] | 93.4 | 84.8 |
Redundancy | 5.4 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | HANGING DROP, pH 6.4, vapor diffusion - hanging drop |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein complex | 5 (mg/ml) | |
2 | 1 | drop | PEG8000 | 15-20 (%(w/v)) | |
3 | 1 | drop | potassium phosphate | 0.1 (M) |