1MEE

THE COMPLEX BETWEEN THE SUBTILISIN FROM A MESOPHILIC BACTERIUM AND THE LEECH INHIBITOR EGLIN-C

Experimental procedure

Spacegroup name	P 1 21 1
Unit cell lengths	42.980, 71.850, 48.340
Unit cell angles	90.00, 110.00, 90.00

Refinement procedure

Resolution	10.000 * - 2.000
R-factor	0.151
RMSD bond length	0.012
RMSD bond angle	0.039
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000 *
High resolution limit [Å]	2.000 *
Rmerge	0.073 *	0.123 *
Total number of observations	32235 *
Number of reflections	17300 *
Completeness [%]	92.3 *	64 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG	18 (%)
2	1	drop	enzyme	5 (mg/ml)
3	1	drop	PEG4000	9 (%)
4	1	drop	PIPES	25 (mM)