1MDT
THE REFINED STRUCTURE OF MONOMERIC DIPHTHERIA TOXIN AT 2.3 ANGSTROMS RESOLUTION
Experimental procedure
Spacegroup name | P 21 21 2 |
Unit cell lengths | 168.500, 135.500, 47.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.300 |
Rwork | 0.207 |
RMSD bond length | 0.022 |
RMSD bond angle | 2.400 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 * | 25 * | microseeding and macroseeding * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 8 (mg/ml) | |
2 | 1 | reservoir | PEG8000 | 17 (%(w/v)) | |
3 | 1 | reservoir | 1.0 (M) | ||
4 | 1 | reservoir | Tris-HCl | 0.05 (M) |