1MDQ

REFINED STRUCTURES OF TWO INSERTION(SLASH)DELETION MUTANTS PROBE FUNCTION OF THE MALTODEXTRIN BINDING PROTEIN

Experimental procedure

Spacegroup name	C 1 2 1
Unit cell lengths	106.010, 66.460, 57.750
Unit cell angles	90.00, 113.17, 90.00

Refinement procedure

Resolution	10.000 - 1.900
R-factor	0.22
Rwork	0.220
RMSD bond length	0.013
RMSD bond angle	0.033 *
Phasing software	X-PLOR
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	1.700 *
Rmerge	0.042 *
Total number of observations	72712 *
Number of reflections	42315
Completeness [%]	78.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.2 *	4 *	reseeded into drops containing 15% PEG8000, taken from Spurlino, J.C. et al (1991). J. Biol. Chem., 266, 5202-5219. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	3.5 (mg/ml)
2	1	drop	maltose	1 (mM)
3	1	drop	PEG8000	16 (%(w/v))
4	1	drop	sodium azide	0.02 (%)
5	1	drop	sodium citrate	10 (mM)
6	1	reservoir	PEG8000	19 (%)