1MDP

REFINED STRUCTURES OF TWO INSERTION(SLASH)DELETION MUTANTS PROBE FUNCTION OF THE MALTODEXTRIN BINDING PROTEIN

Experimental procedure

Spacegroup name	P 1 21 1
Unit cell lengths	55.510, 88.330, 75.790
Unit cell angles	90.00, 95.00, 90.00

Refinement procedure

Resolution	10.000 - 2.300
R-factor	0.187
Rwork	0.187
RMSD bond length	0.009
RMSD bond angle	0.025 *
Phasing software	X-PLOR
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	2.250 *
Rmerge	0.042 *
Total number of observations	62502 *
Number of reflections	39720
Completeness [%]	74.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.5 *		microseeding into drops of 18-20 % PEG over 18-25 % PEG *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	4 (mg/ml)
2	1	drop	maltose	2-3 (mM)
3	1	drop	PEG6000	10-12.5 (%)
4	1	drop	sodium citrate	10 (mM)
5	1	drop	sodium azide	0.02 (%)
6	1	reservoir	PEG	14-16 (%)