1MCE
PRINCIPLES AND PITFALLS IN DESIGNING SITE DIRECTED PEPTIDE LIGANDS
Experimental procedure
Source type | SYNCHROTRON |
Synchrotron site | Photon Factory |
Wavelength(s) | 1.0 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 72.300, 72.300, 185.900 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 6.000 - 2.700 |
R-factor | 0.191 |
RMSD bond length | 0.013 |
RMSD bond angle | 0.028 |
Phasing software | X-PLOR |
Refinement software | PROLSQ |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Batch method * | 6.2 * | THIS COMPLEX WAS PREPARED BY DIFFUSION OF THE PEPTIDE INTO A CRYSTAL OF THE DIMER. |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | sodium pshosphate | 0.2 (M) | |
2 | 1 | 1 | protein | 23 (mg/ml) | |
3 | 1 | 1 | ammonium sulfate | 1.6-1.9 (M) |