1MC4
Crystal Structure of Aspartate-Semialdehyde dehydrogenase from Vibrio Cholerae El Tor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2001-09-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 43 3 2 |
| Unit cell lengths | 141.877, 141.877, 141.877 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.350 - 2.770 |
| Rwork | 0.215 |
| R-free | 0.28100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mb4 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.870 | 2.870 |
| High resolution limit [Å] | 2.770 | 2.770 |
| Rmerge | 0.054 | 0.054 |
| Number of reflections | 24324 | |
| <I/σ(I)> | 33.53 | 9.26 |
| Completeness [%] | 99.1 | 99.5 |
| Redundancy | 31.9 | 10.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 20% PEG 3350, 0.2M Sodium Acetate, 0.1M Tris pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
