1MC3
CRYSTAL STRUCTURE OF RFFH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-10-23 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 144.338, 71.682, 59.413 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.600 |
| Rwork | 0.223 |
| R-free | 0.28400 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 * |
| RMSD bond angle | 1.800 * |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.088 * | |
| Total number of observations | 47293 * | |
| Number of reflections | 16531 | |
| <I/σ(I)> | 9.5 | |
| Completeness [%] | 90.3 | 46.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 291 | PEG8K 16%, 0.1M MES, 0.4M MGCL2, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 18K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris | 20 (mM) | pH7.5 |
| 3 | 1 | drop | 0.2 (M) | ||
| 4 | 1 | drop | dithiothreitol | 5 (mM) | |
| 5 | 1 | drop | dTTP | 5 (mM) | |
| 6 | 1 | reservoir | PEG8000 | 16 (%(w/v)) | |
| 7 | 1 | reservoir | MES | 0.1 (M) | pH6.5 |
| 8 | 1 | reservoir | 0.4 (M) |
