1MC2
monomeric LYS-49 phospholipase A2 homologue purified from AG
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-BM |
Synchrotron site | APS |
Beamline | 17-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-11-10 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.90 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 44.732, 59.086, 45.308 |
Unit cell angles | 90.00, 117.43, 90.00 |
Refinement procedure
Resolution | 20.000 * - 0.800* |
R-factor | 0.0949 |
R-free | 0.12100 |
Structure solution method | AB INITIO |
RMSD bond length | 0.019 |
RMSD bond angle | 1.179 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SnB |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 * | 0.830 |
High resolution limit [Å] | 0.800 * | 0.800 |
Rmerge | 0.055 * | 0.367 * |
Total number of observations | 384478 * | |
Number of reflections | 84242 * | |
<I/σ(I)> | 0.332 | |
Completeness [%] | 76.6 * | 34.7 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 * | 298 | Huang, Q.Q., (2000) Acta Crystallogr., D56, 907. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | 2-propanol | 20 (%(v/v)) | |
2 | 1 | reservoir | PEG4000 | 20 (%(w/v)) | |
3 | 1 | reservoir | sodium citrate | 0.1 (M) | pH5.6 |
4 | 1 | drop | protein | 20-30 (mg/ml) | |
5 | 1 | drop | PEG4000 | 18 (%(w/v)) |