1MBU
Crystal Structure Analysis of ClpSN heterodimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-D |
| Synchrotron site | APS |
| Beamline | 14-BM-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-04-07 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.00930 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 87.913, 87.913, 209.420 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 * - 2.300 |
| Rwork | 0.217 |
| R-free | 0.23600 * |
| Structure solution method | MIR |
| RMSD bond length | 0.012 * |
| RMSD bond angle | 1.460 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.350 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.055 * | 0.579 * |
| Number of reflections | 42650 | |
| <I/σ(I)> | 32.4 | 2.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.31 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 * | 21 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | bis-Tris | 0.1 (M) | pH6.5 |
| 2 | 1 | reservoir | glycerol | 32 (%) | |
| 3 | 1 | reservoir | yttrium chloride | 10-15 (mM) | |
| 4 | 1 | drop | protein | 10 (mg/ml) |
