1MBQ
Anionic Trypsin from Pacific Chum Salmon
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-07-30 |
| Detector | MACSCIENCE DIP100 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.930, 48.010, 82.960 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.169 |
| Rwork | 0.166 |
| R-free | 0.19500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2tbs |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.290 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.067 | 0.183 |
| Total number of observations | 108688 * | |
| Number of reflections | 17253 | |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 4.31 | 4.25 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8.15 | 298 | magnesium sulfate, Tris, calcium chloride, Tris-Cl, benzamidine, pH 8.15, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | CST | 0.62 (mM) | |
| 2 | 1 | drop | 30 (mM) | ||
| 3 | 1 | drop | 0.6 (mM) | ||
| 4 | 1 | drop | benzamidine | 6.0 (mM) | |
| 5 | 1 | drop | Tris-HCl | 50 (mM) | pH8.15 |
| 6 | 1 | reservoir | 1.4 (M) | ||
| 7 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH8.5 |
