1MB8

Crystal Structure of the actin binding domain of plectin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2002-07-24
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	33.597, 79.283, 82.370
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.000 * - 2.150
Rwork	0.212
R-free	0.26100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS (1.1)
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.000 *	2.280
High resolution limit [Å]	2.150	2.150
Rmerge	0.082 *	0.319 *
Total number of observations	42883 *
Number of reflections	11486
Completeness [%]	91.2 *	61.4 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	Tris	0.1 (M)	pH7.0
2	1	reservoir	2-propanol	15 (%(v/v))
3	1	drop	protein	20 (mg/ml)
4	1	drop	Tris	20 (mM)	pH7.0
5	1	drop		150 (mM)