1MB1

MBP1 FROM SACCHAROMYCES CEREVISIAE

Experimental procedure

Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	IMAGE PLATE AREA DETECTOR
Collection date	1997-01
Detector	MARRESEARCH
Spacegroup name	P 41 21 2
Unit cell lengths	42.580, 42.580, 123.680
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.000 - 2.100
R-factor	0.215 *
Rwork	0.215
R-free	0.29000
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	2.470 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CCP4

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	18.000	2.160
High resolution limit [Å]	2.100	2.100
Rmerge	0.041	0.124
Number of reflections	6810
<I/σ(I)>	30	11
Completeness [%]	94.4	93.3
Redundancy	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.9	4 *	Taylor, I.A., (1997) Proteins Struct. Funct. Genet., 27,325. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG3350	28-30 (%(w/v))
2	1	reservoir	Tris-HCl	50 (mM)
3	1	reservoir		200 (mM)