1MAW

Crystal Structure of Tryptophanyl-tRNA Synthetase Complexed with ATP in an Open Conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS II
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	228.550, 91.980, 156.890
Unit cell angles	90.00, 132.34, 90.00

Refinement procedure

Resolution	15.000 - 3.000
Rwork	0.218
R-free	0.23900 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1d2r
RMSD bond length	0.012 *
RMSD bond angle	1.360 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.050
High resolution limit [Å]	3.000 *	3.000 *
Rmerge	0.079 *
Total number of observations	102635 *
Number of reflections	42607
Completeness [%]	87.3 *	70.5 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICRODIALYSIS	6.6	42 *	potassium phosphate, mg-atp, pH 6.6, MICRODIALYSIS, temperature 310K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	4 (mg/ml)
2	1	2	potassium phosphate	2 (M)	pH6.6
3	1	2	ATP	1 (mM)
4	1	2		20 (mM)