1MAI
STRUCTURE OF THE PLECKSTRIN HOMOLOGY DOMAIN FROM PHOSPHOLIPASE C DELTA IN COMPLEX WITH INOSITOL TRISPHOSPHATE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-05-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 59.400, 59.400, 80.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.900 |
| R-factor | 0.205 |
| Rwork | 0.205 |
| R-free | 0.29100 |
| Structure solution method | MIR |
| RMSD bond length | 0.008 |
| RMSD bond angle | 21.420 * |
| Data reduction software | DENZO |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.960 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.100 | |
| Number of reflections | 11978 | |
| <I/σ(I)> | 16.8 | 3.22 |
| Completeness [%] | 99.7 | 99.4 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | pH 6.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein/ligand complex | 1.2 (mM) | |
| 2 | 1 | reservoir | PEG20000 | 12 (%) | |
| 3 | 1 | reservoir | glycerol | 10 (%) | |
| 4 | 1 | reservoir | Na-citrate | 50 (mM) |
