1MAC

CRYSTAL STRUCTURE AND SITE-DIRECTED MUTAGENESIS OF BACILLUS MACERANS ENDO-1,3-1,4-BETA-GLUCANASE

Experimental procedure

Spacegroup name	C 1 2 1
Unit cell lengths	203.680, 42.410, 60.700
Unit cell angles	90.00, 101.27, 90.00

Refinement procedure

Resolution	8.000 - 2.300
R-factor	0.163
RMSD bond length	0.009 *
RMSD bond angle	2.290 *
Refinement software	TNT

Data quality characteristics

	Overall
Low resolution limit [Å]	16.900 *
High resolution limit [Å]	2.300 *
Rmerge	0.106 *
Number of reflections	16928 *
Completeness [%]	72.9 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.3 *		using macroseeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	B.macerans beta-glucanase	6 (mg/ml)
2	1	drop	sodium acetate	10 (mM)
3	1	drop		5 (mM)
4	1	reservoir	ammonium sulfate	30 (%(w/v))
5	1	reservoir		20 (mM)