1MA1
Structure and properties of the atypical iron superoxide dismutase from Methanobacterium thermoautotrophicum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200HB |
| Temperature [K] | 115 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-10-19 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 187.619, 114.082, 58.087 |
| Unit cell angles | 90.00, 94.95, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.600 |
| R-factor | 0.217 |
| Rwork | 0.215 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1SSS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.900 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.550 |
| Rmerge | 0.128 | 0.547 |
| Number of reflections | 34876 | |
| <I/σ(I)> | 7.25 | 1.6 |
| Completeness [%] | 92.9 | 93.2 |
| Redundancy | 9.2 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | PEG 6000, sodium azide, feric chloride, amonium acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
