1M9Z
CRYSTAL STRUCTURE OF HUMAN TGF-BETA TYPE II RECEPTOR LIGAND BINDING DOMAIN
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-07-14 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0092, 1.0011, 1.0076 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.471, 40.658, 76.154 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.000 * - 1.050 |
| R-factor | 0.15647 |
| Rwork | 0.156 |
| R-free | 0.16600 * |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.480 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.000 | 1.090 |
| High resolution limit [Å] | 1.050 | 1.050 |
| Rmerge | 0.035 * | 0.488 * |
| Number of reflections | 45991 * | 2277 * |
| <I/σ(I)> | 56.5 | |
| Completeness [%] | 87.9 | 44.3 |
| Redundancy | 8.5 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | Boesen, C.C., (2002) Acta Crystallogr., D58, 1214. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | reservoir | PEG2000 | 30 (%) | |
| 3 | 1 | reservoir | sodium citrate | 0.1 (M) | pH5.0 |
