1M9U

Crystal Structure of Earthworm Fibrinolytic Enzyme Component A from Eisenia fetida

Replaces:  1IJ7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	CONVENTIONAL Cu
Temperature [K]	291
Detector technology	IMAGE PLATE
Collection date	2000-03-15
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	40.600, 126.100, 129.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	9.000 - 2.300
Rwork	0.191
R-free	0.23600
Structure solution method	MIR
RMSD bond length	0.011
RMSD bond angle	1.630 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	9.000	2.350
High resolution limit [Å]	2.300	2.300
Rmerge	0.162 *	0.586
Number of reflections	29703
<I/σ(I)>	16.2	2.4
Completeness [%]	99.7	98.9
Redundancy	16	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	291	Otwinowski, Z., (1997) Methods Enzymol., 276, 307. *