1M9C
X-ray crystal structure of Cyclophilin A/HIV-1 CA N-terminal domain (1-146) M-type Complex.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1997-07-15 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 38.445, 113.235, 67.006 |
Unit cell angles | 90.00, 100.53, 90.00 |
Refinement procedure
Resolution | 38.000 * - 2.000 |
R-factor | 0.201 |
Rwork | 0.195 |
R-free | 0.25900 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ak4 |
RMSD bond length | 0.018 |
RMSD bond angle | 2.700 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.000 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.063 | 0.195 |
Total number of observations | 89358 * | |
Number of reflections | 31588 | |
<I/σ(I)> | 14.8 | 2.8 |
Completeness [%] | 83.0 | 60 |
Redundancy | 2.8 | 1.55 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 21 * | PEG 8K, Bicine, LiCl, Tris, Beta-mercaptoethanol, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | CypA-CAN | 0.4 (mM) | |
2 | 1 | drop | beta-mercaptoethanol | 1 (mM) | |
3 | 1 | drop | Tris | 10 (mM) | pH8.0 |
4 | 1 | reservoir | PEG8000 | 15-25 (%(w/v)) | |
5 | 1 | reservoir | 1 (M) | ||
6 | 1 | reservoir | Bicine | 100 (mM) | pH7.0-9.0 |