1M90
Co-crystal structure of CCA-Phe-caproic acid-biotin and sparsomycin bound to the 50S ribosomal subunit
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-06-15 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 1.033 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 211.661, 299.773, 573.684 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.800 |
| R-factor | 0.181 |
| Rwork | 0.181 |
| R-free | 0.22600 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jj2 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 15.600 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.136 | 0.900 |
| Number of reflections | 416857 | |
| <I/σ(I)> | 15 | 2 |
| Completeness [%] | 94.3 | 100 |
| Redundancy | 7 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | PEG 6000, NaCl, KCl, NH4Cl, MgCl2, ethylene glycol, pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 100K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 6000 | ||
| 2 | 1 | 1 | NaCl | ||
| 3 | 1 | 1 | KCl | ||
| 4 | 1 | 1 | NH4Cl | ||
| 5 | 1 | 1 | MgCl2 | ||
| 6 | 1 | 1 | ethylene glycol |
