1M8X

CRYSTAL STRUCTURE OF THE PUMILIO-HOMOLOGY DOMAIN FROM HUMAN PUMILIO1 IN COMPLEX WITH NRE1-14 RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	93
Detector technology	IMAGE PLATE
Collection date	2001-03-07
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	262.730, 37.730, 82.520
Unit cell angles	90.00, 102.95, 90.00

Refinement procedure

Resolution	34.290 - 2.200
R-factor	0.236
Rwork	0.236
R-free	0.27400 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1IB3
RMSD bond length	0.007
RMSD bond angle	1.120 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	500.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.083 *	0.343 *
Number of reflections	39467	3718 *
<I/σ(I)>	12.3	2.1
Completeness [%]	96.6	93.2
Redundancy	3	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4 *	20 *	PEG 3350, lithium sulfate, sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5.5 (mg/ml)
2	1	drop	Tris	10 (mM)	pH7.4
3	1	drop		150 (mM)
4	1	drop	dithiothreitol	2 (mM)
5	1	reservoir	PEG3350	14 (%(w/v))
6	1	reservoir		100 (mM)
7	1	reservoir	sodium citrate	100 (mM)	pH5.6