1M8U
Crystal Structure of Bovine gamma-E at 1.65 Ang Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-06-13 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9797 |
| Spacegroup name | P 1 |
| Unit cell lengths | 30.800, 36.710, 46.250 |
| Unit cell angles | 100.06, 106.92, 105.27 |
Refinement procedure
| Resolution | 20.000 - 1.650 |
| Rwork | 0.186 |
| R-free | 0.21600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a5d |
| RMSD bond length | 0.050 |
| RMSD bond angle | 1.280 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.000 | 1.740 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Number of reflections | 20771 | |
| <I/σ(I)> | 8 | 4.4 |
| Completeness [%] | 92.0 | 79.1 |
| Redundancy | 2.2 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 4.5 | 277 | PEG 3000, sodium acetate, pH 4.5, microbatch, temperature 277K |
