1M8N
Choristoneura Fumiferana (Spruce Budworm) Antifreeze Protein Isoform 501
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2001-01-15 |
Detector | MARRESEARCH |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 43.900, 57.400, 70.130 |
Unit cell angles | 90.00, 92.10, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.450 |
Rwork | 0.221 |
R-free | 0.29100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Theoretical model based on pdb 1L0S |
RMSD bond length | 0.006 * |
RMSD bond angle | 1.300 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | EPMR |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.600 |
High resolution limit [Å] | 2.450 | 2.450 |
Rmerge | 0.078 * | 0.356 * |
Total number of observations | 567512 * | |
Number of reflections | 12991 * | |
Completeness [%] | 85.7 * | 71.3 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 295 | PEG-4000, iso-propanol, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG4000 | 18 (%) | |
2 | 1 | reservoir | isopropanol | 20 (%) | |
3 | 1 | reservoir | sodium citrate | 100 (mM) | pH4.8-5.6 |