1M8K
Crystal Structure Of Methanobacterium Thermoautotrophicum Nicotinamide Mononucleotide Adenylyltransferase Mutant H19A complexed with NAD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-09-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 124.480, 124.480, 111.826 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 * - 3.000 |
| R-factor | 0.194 |
| Rwork | 0.194 |
| R-free | 0.23300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ej2 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 22.000 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.140 | 0.393 |
| Total number of observations | 223204 * | |
| Number of reflections | 20207 | |
| <I/σ(I)> | 20.6 | 6.5 |
| Completeness [%] | 100.0 | 100 * |
| Redundancy | 11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 20 * | 1.5 M Ammonium Sulfate, 100 mM Tris, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 1.6 (M) | |
| 2 | 1 | reservoir | glycerol | 5 (%) | |
| 3 | 1 | reservoir | Tris-HCl | 100 (mM) | pH8.0 |
| 4 | 1 | drop | ammonium sulfate | 1.5-1.6 (M) | |
| 5 | 1 | drop | glycerol | 5 (%) | |
| 6 | 1 | drop | Tris-HCl | 100 (mM) | pH8.0 |
| 7 | 1 | drop | protein | 10 (mg/ml) |
