1M7X

The X-ray Crystallographic Structure of Branching Enzyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	116
Detector technology	CCD
Collection date	2000-03-23
Detector	CUSTOM-MADE
Wavelength(s)	0.97794
Spacegroup name	P 1 21 1
Unit cell lengths	91.475, 102.619, 185.060
Unit cell angles	90.00, 91.45, 90.00

Refinement procedure

Resolution	35.000 - 2.300
Rwork	0.200
R-free	0.26500 *
Structure solution method	SAD, SIR
RMSD bond length	0.007 *
RMSD bond angle	1.450 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.086 *	0.303
Number of reflections	151550
<I/σ(I)>		2.6
Completeness [%]	99.6	98.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	277	Hepes, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	277	Hepes, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	277	Hepes, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	sodium HEPES	25 (mM)	pH7.5
2	1	drop	protein	5 (mg/ml)
3	1	reservoir	sodium HEPES	100 (mM)	pH7.20