1M7J
Crystal structure of D-aminoacylase defines a novel subset of amidohydrolases
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-04-10 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.102, 77.167, 135.744 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 150.000 * - 1.500 |
| R-factor | 0.161 |
| Rwork | 0.160 |
| R-free | 0.17400 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.770 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 150.000 * | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.047 | 0.116 |
| Number of reflections | 100783 | 9984 * |
| <I/σ(I)> | 50.5 | 17.6 |
| Completeness [%] | 99.2 | 100 |
| Redundancy | 6.3 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 295 | Hsu, C.-S., (2002) Acta Crystallogr., Sect.D, 58, 1482. * |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 295 | Hsu, C.-S., (2002) Acta Crystallogr., Sect.D, 58, 1482. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 25-30 (%) | |
| 2 | 1 | reservoir | sodium citrate | 0.1 (M) | pH5.6 |
| 3 | 1 | reservoir | ammonium acetate | 0.2 (M) | |
| 4 | 1 | drop | protein | 12-20 (mg/ml) |
