1M71

Crystal structure of a Monoclonal Fab Specific for Shigella Flexneri Y lipopolysaccharide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	281
Detector technology	DIFFRACTOMETER
Collection date	1990-06-02
Detector	WEISSENBERG
Wavelength(s)	1.0
Spacegroup name	P 43 21 2
Unit cell lengths	70.700, 70.700, 202.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.760 - 2.800
Rwork	0.223
R-free	0.29200 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mpc
RMSD bond length	0.007 *
RMSD bond angle	1.400 *
Data reduction software	WEIS
Data scaling software	WEIS
Phasing software	MERLOT
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.970
High resolution limit [Å]	2.800	2.800
Rmerge	0.116
Total number of observations	108812 *
Number of reflections	10392
<I/σ(I)>	5
Completeness [%]	77.9	59.9
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	277	Vyas, M.N., (1993) J. Mol. Biol., 231, 133. *