1M70

Crystal structure of oxidized recombinant cytochrome c4 from Pseudomonas stutzeri

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	2000-12-07
Detector	MARRESEARCH
Wavelength(s)	1.54179
Spacegroup name	P 1 21 1
Unit cell lengths	74.960, 57.640, 84.410
Unit cell angles	90.00, 104.23, 90.00

Refinement procedure

Resolution	30.000 - 1.250
R-factor	0.1571
Rwork	0.155
R-free	0.20210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1etp
RMSD bond length	0.011
RMSD bond angle	0.028
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.290
High resolution limit [Å]	1.250	1.250
Number of reflections	175219
Completeness [%]	90.9	77

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.6	298	0.2M ammonium acetate, 0.1M sodium citrate pH 5.6, 30%(w/v) PEG 4000, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	6.6	298	0.2M ammonium acetate, 0.1M sodium citrate pH 5.6, 30%(w/v) PEG 4000, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K