1M6X
Flpe-Holliday Junction Complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-06-04 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 79.779, 116.511, 142.425 |
Unit cell angles | 90.00, 97.26, 90.00 |
Refinement procedure
Resolution | 500.000 * - 2.800 |
R-factor | 0.239 |
Rwork | 0.239 |
R-free | 0.28600 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1flo |
RMSD bond length | 0.007 |
RMSD bond angle | 1.324 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | EPMR |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.500 | 3.000 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 48174 | |
Completeness [%] | 18.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 4 * | PEG 5Kmme, sodium cloride, calcium cloride, HEPES, xylitol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 6 (mg/ml) | |
10 | 1 | reservoir | dithiothreitol | 2 (mM) | |
2 | 1 | drop | 100 (mM) | ||
3 | 1 | drop | HEPES | 10 (mM) | pH7.0 |
4 | 1 | drop | dithiothreitol | 2 (mM) | |
5 | 1 | reservoir | PEG5000 MME | 13 (%(w/v)) | |
6 | 1 | reservoir | HEPES | 20 (mM) | pH7.0 |
7 | 1 | reservoir | 100 (mM) | ||
8 | 1 | reservoir | 10 (mM) | ||
9 | 1 | reservoir | xylitol | 20 (%(w/v)) |