1M6P

EXTRACYTOPLASMIC DOMAIN OF BOVINE CATION-DEPENDENT MANNOSE 6-PHOSPHATE RECEPTOR

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	1997-04
Detector	RIGAKU RAXIS II
Spacegroup name	P 43 21 2
Unit cell lengths	92.641, 92.641, 85.316
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.800
R-factor	0.224
Rwork	0.224
R-free	0.27600
Structure solution method	MULTIPLE ISOMORPHOUS REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	26.100 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASES
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	1.820
High resolution limit [Å]	1.800	1.800
Rmerge	0.077 *	0.340
Total number of observations	144591 *
Number of reflections	31187 *
<I/σ(I)>	10.7	2
Completeness [%]	89.0	72.5
Redundancy	4.6	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	6.6 *	19 *	pH 6.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8.8 (mg/ml)
2	1	reservoir	ammonium acetate	0.20 (M)
3	1	reservoir	PEG4000	25 (%)
4	1	reservoir	cacodylate	0.10 (M)	pH6.6