1M6G
Structural Characterisation of the Holliday Junction TCGGTACCGA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-05-19 |
Detector | MARRESEARCH |
Wavelength(s) | 0.811 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 64.302, 25.034, 36.778 |
Unit cell angles | 90.00, 110.04, 90.00 |
Refinement procedure
Resolution | 34.503 - 1.652 |
R-factor | 0.2148 |
Rwork | 0.215 |
R-free | 0.29566 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.017 |
RMSD bond angle | 2.868 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | REFMAC (5.1.19) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.503 | 1.695 |
High resolution limit [Å] | 1.649 | 1.652 |
Rmerge | 0.296 | 0.332 |
Number of reflections | 6703 | |
Redundancy | 5.41 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 290 | cacodylate, strontium chloride, MPD, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | cacodylate | ||
2 | 1 | 1 | SrCl2 | ||
3 | 1 | 1 | MPD |