1M6C

V68N MYOGLOBIN WITH CO

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	150
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS
Spacegroup name	I 1 21 1
Unit cell lengths	122.140, 42.300, 91.550
Unit cell angles	90.00, 92.49, 90.00

Refinement procedure

Resolution	24.000 - 1.900
R-factor	0.192 *
Rwork	0.192
R-free	0.26100
Structure solution method	OTHER
RMSD bond length	0.019
RMSD bond angle	0.037
Data reduction software	DENZO
Data scaling software	CCP4
Phasing software	CCP4
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.000
High resolution limit [Å]	1.900
Rmerge	0.042 *	0.214 *
Number of reflections	35786
<I/σ(I)>	22.3
Completeness [%]	94.7 *
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.1		0.1 PHOSPHATE BUFFER PH 7.1 10 MG/ML PROTEIN

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	ammonium sulfate	72-80 (%(v/v)sat)
2	1	drop	phosphate	0.1 (M)
3	1	drop	protein	10 (mg/ml)