1M61
Crystal structure of the apo SH2 domains of ZAP-70
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 200 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-05-06 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0158 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.851, 52.227, 54.455 |
| Unit cell angles | 90.00, 89.91, 90.00 |
Refinement procedure
| Resolution | 18.850 - 2.500 |
| Rwork | 0.245 |
| R-free | 0.28800 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a81 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.390 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNX (2002) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.062 * | 0.456 * |
| Number of reflections | 9426 | |
| <I/σ(I)> | 10.2 | 1.7 |
| Completeness [%] | 98.1 | 97.8 |
| Redundancy | 4.2 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 * | 298 | PEG3350, Sodium chloride, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | sodium phosphate | 100 (mM) | pH6.80 |
| 3 | 1 | drop | 50 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 5 (mM) | |
| 5 | 1 | reservoir | PEG3350 | 20 (%) | |
| 6 | 1 | reservoir | HEPES | 0.1 (M) | pH8. |
| 7 | 1 | reservoir | 0.2 (M) | ||
| 8 | 1 | reservoir | glutathione | 20 (mM) | |
| 9 | 1 | reservoir | PEG3350 | 15 (%) |
