1M5Y

Crystallographic Structure of SurA, a Molecular Chaperone that Facilitates Outer Membrane Porin Folding

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2002-01-01
Detector	ADSC QUANTUM 315
Wavelength(s)	0.965
Spacegroup name	F 2 2 2
Unit cell lengths	158.817, 223.406, 279.721
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 * - 3.000
R-factor	0.228
Rwork	0.228
R-free	0.28300
RMSD bond length	0.008
RMSD bond angle	1.380 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.050
High resolution limit [Å]	3.000	3.000
Rmerge	0.077 *	0.348 *
Total number of observations	217260 *
Number of reflections	46785 *
<I/σ(I)>	1.62	3.9
Completeness [%]	92.9 *	85.2
Redundancy	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	18 *	0.75M ammonium sulphate, 100mM sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP at 303K, VAPOR DIFFUSION, HANGING DROP, temperature 303.0K
1	VAPOR DIFFUSION, HANGING DROP	5.6	18 *	0.75M ammonium sulphate, 100mM sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP at 303K, VAPOR DIFFUSION, HANGING DROP, temperature 303.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	0.75 (M)
2	1	reservoir	sodium citrate	100 (mM)	pH5.6