1M5U
CRYSTAL STRUCTURE OF THE RESPONSE REGULATOR DIVK. STRUCTURE AT PH 8.0 IN THE APO-FORM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2000-04-17 |
Detector | MARRESEARCH |
Wavelength(s) | 0.980 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 36.670, 40.710, 66.290 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 1.870 |
R-factor | 0.1901 |
Rwork | 0.190 |
R-free | 0.20700 * |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | APO-DIVK SOLVED AT PH6 |
RMSD bond length | 0.005 |
RMSD bond angle | 22.600 * |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 1.970 |
High resolution limit [Å] | 1.870 | 1.870 |
Rmerge | 0.037 | 0.097 |
Total number of observations | 42648 * | |
Number of reflections | 8414 | |
<I/σ(I)> | 11.3 | 7.9 |
Completeness [%] | 97.9 | 94.1 |
Redundancy | 5.1 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6 * | 285 | Cabantous, S., (2002) Acta Crystallogr., Sect.D, 58, 1249. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 2 (mg/ml) | |
2 | 1 | drop | MES/NaOH | 20 (mM) | pH6.0 |
3 | 1 | drop | dithiothreitol | 5 (mM) | |
4 | 1 | reservoir | PEG550 MME | 32 (%) | |
5 | 1 | reservoir | MES | 40 (mM) | pH6.0 |
6 | 1 | reservoir | dithiothreitol | 5 (mM) | |
7 | 1 | reservoir | 0.01 (%) |