1M5B
X-RAY STRUCTURE OF THE GLUR2 LIGAND BINDING CORE (S1S2J) IN COMPLEX WITH 2-Me-Tet-AMPA AT 1.85 A RESOLUTION.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-03-31 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 114.410, 164.330, 47.310 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.850 |
| Rwork | 0.196 |
| R-free | 0.23400 |
| Structure solution method | Difference Fourier. |
| Starting model (for MR) | 1FTM(S1S2J-AMPA molecule A). |
| RMSD bond length | 0.005 |
| RMSD bond angle | 21.600 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (0.5) |
| Refinement software | CNS (0.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.970 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.063 | 0.284 |
| Number of reflections | 76427 | |
| <I/σ(I)> | 19 | 2.4 |
| Completeness [%] | 99.0 | 91.4 |
| Redundancy | 4.4 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 4 * | PEG 8000, Zn(OAc)2, cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 279K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | HEPES | 10 (mM) | pH7.0 |
| 3 | 1 | drop | 20 (mM) | ||
| 4 | 1 | drop | EDTA | 1 (mM) | |
| 5 | 1 | drop | (S)-2-Me-Tet-AMPA | 3 (mM) | |
| 6 | 1 | reservoir | PEG8000 | 12-18 (%(w/v)) | |
| 7 | 1 | reservoir | zinc acetate | 0.15-0.28 (M) | |
| 8 | 1 | reservoir | sodium cacodylate | 0.1 (M) | pH6.5 |
