1M4Z

Crystal structure of the N-terminal BAH domain of Orc1p

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Detector technology	CCD
Collection date	2000-01-01
Detector	BRANDEIS - B4
Wavelength(s)	1.1
Spacegroup name	P 21 21 2
Unit cell lengths	128.260, 61.050, 67.770
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.200
R-factor	0.225
Rwork	0.200
R-free	0.22500
Structure solution method	MAD
Data scaling software	SCALEPACK
Phasing software	PHASES
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.240
High resolution limit [Å]	2.200	2.200
Rmerge	0.042	0.111
Number of reflections	26610
<I/σ(I)>	19.5	5.6
Completeness [%]	95.2	84
Redundancy	11.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	16 *	PEGMME 2000, magnesium acetate, magnese chloride, MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	Tris	0.1 (M)	pH7.5
2	1	reservoir	PEG2000 MME	28 (%)
3	1	reservoir	magnesium acetate	0.2 (M)
4	1	reservoir		10 (mM)
5	1	reservoir	MPD	10 (%)
6	1	drop	protein	30 (mg/ml)